Binding of 99mTc to human serum albumin

Binding of 99mTc to human serum albumin

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The specificity of 99m Tc-MDP binding to HSA was examined , the results showed that the non specific binding was 21-23% of the total binding.The range of optimum pH for the binding was 7.4-7.6.

The most suitable amounts for HSA to give maximum binding was (70mg)per incubation medium , binding redken shades 9gi seemed to be affected by 99m Tc- MDP concentration and also mono and divalent metal chloride salts.The association constants (ka) and maximum binding capacity (Bmax) of 99m Tc-MDP binding to its binding sites of HSA was determined from scatchard and double nightstick twm-850xl reciprocal plots , the results revealed that (ka) and (Bmax) values were temperature dependant.